Fluorescence imaging articles from across Nature Portfolio |

Fluorescence imaging is the visualization of fluorescent dyes or proteins as labels for molecular processes or structures. 

It enables a wide range of experimental observations including the location and dynamics of gene expression, protein expression and molecular interactions in cells and tissues.

Latest Research and Reviews

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  Near-infrared fluorogenic RNA for in vivo imaging and sensing

  Most of the available fluorogenic RNA approaches emit visible fluorescence, and there is a need for tools working at longer wavelengths. Here, the authors identify and modulate red fluorescent protein-like fluorophores to bind the fluorogenic RNA Squash, thereby obtaining near-infrared fluorogenic RNA-based tools.

  • Zhenyin Chen
  • Wei Chen
  • Xing Li

  ResearchOpen Access09 Jan 2025Nature Communications

  Volume: 16, P: 518
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  Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells

  The spatial distribution of glycosylated RNAs in single cells can be imaged using a protocol that uses an aptamer and a DNA probe that are specific for sialic acid-containing glycosylated RNAs. It can be applied to imaging other glycosylated RNAs using aptamers for other glycans.

  • Weijie Guo
  • Yuan Ma
  • Yi Lu

  Protocols08 Jan 2025Nature Protocols

  P: 1-21
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  Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy

  Optical aberrations can hinder fluorescence imaging of thick samples, reducing image signal, contrast, and resolution. Here, the authors introduce a deep learning-based strategy for aberration compensation, improving image quality without slowing image acquisition or introducing more optics.

  • Min Guo
  • Yicong Wu
  • Hari Shroff

  ResearchOpen Access02 Jan 2025Nature Communications

  Volume: 16, P: 313
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  Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy

  Combining expansion microscopy (ExM) with lightsheet imaging can enable fast 3D visualisation of biological structures at high-resolution, but such approaches can be hindered by several limitations. By using potassium acrylate-based hydrogels, the authors perform large-volume ExM with Bessel lightsheet microscopy, achieving high-resolution imaging of cellular structures within the fly brain.

  • Xuejiao Tian
  • Tzu-Yang Lin
  • Bi-Chang Chen

  ResearchOpen Access30 Dec 2024Nature Communications

  Volume: 15, P: 10911
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  Expression of Yes-associated protein in endothelial cells of human corneas before and after storage in organ culture

  • Hanielle Vaitinadapoulé
  • Olfa Ben Moussa
  • Frédéric Mascarelli

  ResearchOpen Access28 Dec 2024Scientific Reports

  Volume: 14, P: 31073
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  Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy

  Detection of fortunate molecules by correlation-based single-molecule localization microscopy enhances signal-to-background ratio and improves spatial resolution, leading to better resolved single-molecule ensembles in a cellular system.

  • Aravinth S
  • Francesca Cella Zanacchi
  • Partha Pratim Mondal

  ResearchOpen Access23 Dec 2024Communications Biology

  Volume: 7, P: 1693

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